A children's ministry held at Wagnall's Memorial in Lithopolis, Ohio, was the impetus for the establishment of the Greencastle Bible Church. Several children were converted at these Bible studies and reported this good news to their parents. These families saw a need for a Bible-teaching and preaching church in Bloom Township. In September of 1965, a small group of believers founded Greencastle Bible Church and began meeting at the Township Building of Bloom Township. The Reverend Gordon Ferrell was called as the first pastor.
In 1966, ground was broken for the first building located on Amanda-Northern Road. During the early years of the church, Pastor Ferrell saw the Lord call many of the youth into full-time Christian ministry.
Upon Pastor Ferrell's retirement in 1971, the congregation called Pastor Robert Mazzie. During Pastor Mazzie's time, the church experienced solid and substantial growth. In 1977, the congregation started the Greencastle Christian School. The current auditorium was completed during Pastor Mazzie's tenure.
Pastor Charles Flesher became Greencastle's third pastor in June of 1978. A new educational wing and a multipurpose building were added during Pastor Flesher's ministry. In 1998, the congregation elected Pastor Paul Martin as Associate Pastor. In December of 2010, Pastor Martin became Associate Emeritus. Pastor Flesher transitioned to his position as Pastor Emeritus in July of 2016.
Beginning in the summer of 2016, Pastor Troy Lohmeyer served as Greencastle's fourth pastor.
Pastor Michael Wyatt became Greencastle's Senior Pastor in June of 2020.
Greencastle is affiliated with the Ohio Bible Fellowship, a group of churches which has a large and thriving camping ministry 50 miles north of Columbus. Greencastle Bible Church is noted for its efforts to build strong families, maintain sound standards of Christian conduct, and emphasize excellent music. The theme song of Greencastle is "To God Be the Glory." It is the desire of this fellowship to give God all the glory for the wonderful things He has done in this local church.